Rapid nutritional regulation of oligodendrocytes of the adult hypothalamic median eminence revealed by single-cell transcriptomic analysis

The median eminence (ME) of the hypothalamus is a structure that rapidly adapts to nutrient availability. We have used single-cell RNA sequencing to characterize the transcriptional profiles of cells specifically in the median eminence in the fasted and refed states. In our study, we focus on characterizing the oligodendrocyte population of the ME, and identifying how subpopulations respond to nutrient availability. Overall design: We sequenced 5982 cells from the median eminence of the hypothalamus of fasted and refed mice. RNA profiles were used to characterize cells in this region and identify changes in genes and pathways between experimental conditions.