Nanopore direct RNA sequencing of mRNAs obtained from sorted nuclei from mouse brain. Mice were treated with extintion learning (EXT) or not treated (RC).

NIAID Data Ecosystem00

Tamoxifen induction of Cre recombinase does not cause long lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies (RNA-Seq).

他莫昔芬诱导Cre重组酶系统

神经细胞转录组学

The systemic delivery of tamoxifen (Tam) to activate inducible CreERT2-loxP transgenic mouse systems is now widely used in neuroscience studies. This critical technological advancement allows temporal

NIAID Data Ecosystem00

Supplemental Figure 1. Knockdown effect of Toll and spz genes.

基因功能研究

基因表达调控

Supplemental Figure 1. Knockdown effect of Toll and spz genes. Effects of knockdown of the Toll (A)-(E) and spz (F) genes by RNAi were examined by qRT-PCR. On day 0, pupae were inject

Figshare2023-01-12 更新30

The paired primers used for gene cloning, disruption, complement, silencing and expression.

基因功能研究

分子克隆

*H = A, C or T; B = C, G or T; V = A, C or G; R = A or G. The enzyme sites are italicized while the gateway fragments for exchange are in bold.

Figshare2015-12-02 更新00