Comparing gene-expression profiles between control and CHD6 knock-down HCT116 cells. Comparing gene-expression profiles between control and CHD6 knock-down HCT116 cells

Colorectal cancer is one of the most common cancers. Cancer cells are highly dependent on dysregulated gene expression to support their uncontrolled growth and high energy needs. The expression of gene encoding chromodomain helicase DNA binding protein 6 (CHD6) is frequently altered in tumor tissues of colorectal cancer patients.We performed transcriptomic analysis to identify the differentially expressed genes between control and CHD6 KD HCT116. Overall design: Colorectal cancer cell line HCT116 with Tet-on inducible CHD6 KD construct was treated with doxycycline or vehicle for 48h. And cells were collected for RNA extraction. The RNA samples were hybridized on Affymetrix microarrays.