Cellular transcriptome upon HSV-1 infection or DUX4 overexpression

We found that the germline transcription factor double homeobox 4 (DUX4) is upregulated upon infection with wild-type herpes simplex virus-1 (HSV-1). The goal of this experiment was to compare the cellular transcriptome of HEK293T cells that were infected with HSV-1 (KOS strain), or transfected with a plasmid encoding human DUX4. Overall design: HEK293T cells remained either untreated (mock), were infected with wild-type HSV-1 (KOS strain) for 18 h, or were transiently transfected with a plasmid encoding untagged human DUX4 (pmkg-DUX4) for 18 h, followed by extraction of total RNA and RNAsequencing.