27f/1392r sheared soil amplicons for EMIRGE
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We extracted DNA from 6 soils and used the 27f/1392r primer pair to amplify near full-length 16S rRNA genes. The amplicons were sheared using the Covaris M220 (Covaris, Woburn, MA) and gene libraries were prepared using TruSeq DNA LT library preparation kits (Illumina, San Diego, CA). Samples were pooled and sequenced on an Illumina MiSeq (2x300bp) at the University of Colorado Next Generation Sequencing Facility.<br>We used these raw sequences to re-assemble the near full length 16S rRNA amplicon using EMIRGE.
创建时间：
2016-09-02
