RBM15B Regulates progression of MLL-rearranged leukemia

We identified RBM15B that promotes progression of MLL-r leukemia. In this study, we preformed the m6A RIP-seq, RNA-seq and RBM15B CUT& Tag sequensing to found that RBM15B selectively regulated m6A modified region in the 5' UTR and in part of the CDS around the start codon (two wings around start codon (TWINS)) of mRNA transcribed from target genes of MLL-fusion proteins, which is guided by histone modification H3K79me2. Overall design: 1. epz5676 and DMSO treated MOLM-13 cells used in the m6A RIP-seq and RBM15B CUT& Tag sequensing.2. Sh-NC and sh-RBM15 MOLM-13 cells used.