Genome-wide analysis of mRNA cleavage sites in in Arabidopsis thaliana using expanded leaf

In a previous study, a high-throughput approach for genome-wide profiling of RNA cleavage sites was developed (e.g. Parallel analysis of RNA end (PARE), 5'P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3' transcripts; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) in Arabidopsis thaliana (expanded leaf, three older, non-cotyledon leaves were collected from 21days after germination) to determine the truncated 5' RNA ends (cleavage sites) and analyze the sequence feature around the cleavage sites.