RNA-seq of TFCP2L1 KO and Control Cells

This RNA-seq study aims to investigate the transcriptomic changes associated with TFCP2L1 knockout in Ishikawa cells, a well-characterized human endometrial epithelial cell line, in comparison with wild-type control cells. TFCP2L1 is a key regulator of endometrial receptivity and embryo implantation, and its deficiency is linked to recurrent implantation failure (RIF) in assisted reproductive technology. By profiling the global gene expression differences between TFCP2L1-knockout and control cells, this study seeks to identify dysregulated signaling pathways and molecular targets downstream of TFCP2L1, with a specific focus on the GSK3beta/beta-catenin pathway that mediates endometrial receptivity. The transcriptomic data generated here will elucidate the molecular mechanisms by which TFCP2L1 modulates endometrial epithelial cell function, provide novel insights into the pathological basis of RIF, and lay a foundation for developing targeted therapeutic strategies to improve embryo implantation success rates in clinical practice.