Role of small RNA in post-natal olfactory bulb neurogenesis in mouse

We performed deep-sequencing analysis of small RNA extracted from neuronal progenitors at different developmental stages. The RNA samples were extracted from microdissected tissues of dorsal or lateral sub-ventricular zone (SVZ) (to analyze the immature progenitor pools), of rostral migration stream (RMS) (to analyze the migrating neuroblasts) of olfactory bulbs (OB) (to analyze both immature and mature neurons). These tissues were dissected from animals at variable ages: P1 and P6 for SVZ samples, P15 and P28 for RMS and OB samples. Expression profile of microRNA in time and space along post-natal neurogenesis本研究对不同发育阶段的神经前体细胞所提取的小RNA（small RNA）开展了深度测序分析。所用RNA样本均来自显微切割的三类组织：其一为背侧或外侧室下区（sub-ventricular zone, SVZ），用于分析未成熟前体细胞库；其二为嗅球（olfactory bulbs, OB）的嘴侧迁移流（rostral migration stream, RMS），用于分析迁移中的成神经细胞；其三为嗅球（OB）组织本身，可同时用于分析未成熟与成熟神经元。上述组织分别取自不同日龄的实验动物：SVZ样本取自出生后第1天（P1）与第6天（P6）的个体，RMS样本及OB组织样本则取自出生后第15天（P15）与第28天（P28）的个体。本数据集涵盖了产后神经发生过程中微小RNA（microRNA）的时空表达谱特征。