Genome wide study of microglia in Specific-pathogen-free (SPF) and Germ-free (GF) mice at different stages in males and females (RNA-Seq)

RNAseq was performed on microglia from male and female, SPF or GF mice to elucidate the genetic differences implicated by microbiota and gender. DEGs between the various groups gave some ideas on what different pathways or functions might be affected due to the different factors. Microglia from SPF and GF mice from embryonic and adult stages of both gender were sorted for sequencing. DEGs were obtained to observe if any significant genes were affected. Pathway analysis was performed with the set of DEGs.本研究针对雄性、雌性无特定病原体（Specific Pathogen Free, SPF）小鼠与无菌（Germ Free, GF）小鼠的小胶质细胞开展RNA测序（RNA-seq），以阐明微生物群与性别所介导的遗传差异。不同组别间的差异表达基因（Differentially Expressed Genes, DEGs）分析可为明确不同因素所影响的信号通路及生物学功能提供参考思路。本研究分选了雌雄胚胎期与成年期SPF及GF小鼠的小胶质细胞用于测序。通过分析获取差异表达基因，以观测是否存在受相关因素显著影响的基因。针对所得差异表达基因集开展通路分析。