Gap-free telomere-to-telomere genome assembly of marbled flounder (Pseudopleuronectes yokohamae)

Here, we reported the first gap-free T2T genome in flatfish (marbled flounder), with length of 582.95 Mb combing short reads, PacBio HiFi long reads, ONT ultra-long reads, and Hi-C data. All of the genome sequences were assembled onto 24 chromosomes, and 48 telomeres were identified on both ends of all chromosomes. 99.29% complete BUSCOs were identified, demonstrating a high level of completeness. The average mapping ratio of short reads, PacBio HiFi reads, and ONT ultra-long reads aligned to the genome assembly was more than 99.89%. Moreover, 56.54 Mb repeating elements and 22,778 protein-coding genes were identified in the genome assembly. These results provide valuable resources for the genetic research and the development of molecular breeding technologies in marbled flounder.