Sporulation is decreased in a Δmas mutant.
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Spores were harvested from a TPM plate after 5 days of growth at 32°C and resuspended in 100 µl of TPM buffer. After 2 h incubation at 50°C, spore aliquots were diluted and plated on CTPM agar. After 3 days incubation at 32°C, CFU's from heat resistant spores were counted. All values are given as a percentage of the WT level, normalized to 100%.
创建时间:
2015-12-02



