Table_12_Effects of Lysophosphatidylcholine on Jejuna Morphology and Its Potential Mechanism.XLSX

Lysophosphatidylcholine (LPC) plays a vital role in promoting jejuna morphology in broilers. However, the potential mechanism behind LPC improving the chicken jejuna morphology is unclear. Therefore, the present study was designed to reveal the important genes associated with LPC regulation in birds' jejuna. Thus, GSE94622, the gene expression microarray, was obtained from Gene Expression Omnibus (GEO). GSE94622 consists of 15 broiler jejuna samples from two LPC-treated (LPC500 and LPC1000) and the control groups. Totally 98 to 217 DEGs were identified by comparing LPC500 vs. control, LPC1000 vs. control, and LPC1000 vs. LPC500. Gene ontology (GO) analysis suggested that those DEGs were mainly involved in the one-carbon metabolic process, carbon dioxide transport, endodermal cell differentiation, the positive regulation of dipeptide transmembrane transport, cellular pH reduction, and synaptic transmission. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated the DEGs were enriched in NOD-like receptor (NLR), RIG-I-like receptor (RILR), Toll-like receptor (TLR), and necroptosis signaling pathway. Moreover, many genes, such as RSAD2, OASL, EPSTI1, CMPK2, IFIH1, IFIT5, USP18, MX1, and STAT1 might be involved in promoting the jejuna morphology of broilers. In conclusion, this study enhances our understanding of LPC regulation in jejuna morphology.

溶血磷脂酰胆碱（LPC）在促进肉鸡回肠形态发生中发挥着至关重要的作用。然而，LPC改善鸡回肠形态的潜在机制尚不明确。因此，本研究旨在揭示与鸟类回肠中LPC调控相关的重要基因。鉴于此，我们从基因表达综合数据库（GEO）获取了基因表达微阵列GSE94622。GSE94622包含来自两组LPC处理组（LPC500和LPC1000）及对照组的15个肉鸡回肠样本。通过比较LPC500与对照组、LPC1000与对照组以及LPC1000与LPC500，共鉴定出98至217个差异表达基因（DEGs）。基因本体（GO）分析表明，这些DEGs主要参与单碳代谢过程、二氧化碳转运、内胚层细胞分化、二肽跨膜转运的正向调控、细胞pH降低和突触传递。京都基因与基因组百科全书（KEGG）分析显示，DEGs在NOD样受体（NLR）、RIG-I样受体（RILR）、Toll样受体（TLR）和细胞焦亡信号通路中富集。此外，许多基因，如RSAD2、OASL、EPSTI1、CMPK2、IFIH1、IFIT5、USP18、MX1和STAT1可能参与促进肉鸡回肠形态的形成。总之，本研究加深了我们对回肠形态中LPC调控机制的理解。