Mammalian circRNA Atlas

We developed a new pipeline for accurate circRNA detection. We build a new atlas for characterizing circRNA in human, monkey, mouse, rat and drosophila brain. Our approach allows full annotation of circRNAs with fewer false positives and negatives than any individual pipeline or combination of them. Moreover, our approach is more sensitive than any individual pipeline and allows more accurate quantification and larger number of differentially expressed circRNAs. Overall design: We obtained commercial purified RNA and performed rRNA depletion. Then we treated total RNA with RNAseR and prepared a library.