Genome-wide analysis of ATF4 binding in histidinol-treated cells. Homo sapiens

Activating Transcription Factor 4 (ATF4) is a transcription factor induced by the integrated stress response (ISR). This experiment is a genome-wide occupancy profiling of ATF4 in human HAP1 cells. HAP1 is a near-haploid human cell line that was derived from KBM-7 cells isolated from a patient with Chronic Myelogenous Leukemia. We induced ATF4 expression by mimicking amino acid starvation with the drug histidinol. We identified peaks of ATF4 binding using three independent antibodies. Overall design: Examination of ATF4 binding in HAP1 cells treated with 2 mM histidinol for 24 hours.