Re-examination Characterization and Screening Stripe Rust Resistance Gene of wheat TaPR1 genes based on the Transcriptome in Xinchun 32

Abstract: Pathogenesis-related protein-1 (PR1) encodes a water-soluble protein produced in plants after pathogens infection or abiotic stimulation. It plays a crucial role in plant induced resistance by attacking pathogens, degrading cell wall macromolecules and pathogen toxins, and inhibiting the binding of viral coat proteins to plant receptor molecules. Compared to model plants, the mechanism of action of the PR1 in wheat remains underexplored. In this study, the recently published wheat genome database (IWGSC RefSeq V2.1) was used to identify 83 genes in the TaPR1 gene family. Compared to previous work, remains underexplored. The duplicate genes and corrected misannotated genes previously were removed. Fourteen TaPR1 genes involved in wheat-Pst interaction were identified base on RNA sequencing from XinChun 32. The expression pattern of eight genes were validated using qRT-PCR, and the results showed that PR1 were highly expressed following Puccinia striiformis Westend. f. sp. tritici (Pst) infection. This study enhances previous research on wheat PR1, contributing to a more comprehensive understanding of TaPR1 gene family and providing a reference for screening more broad-spectrum and high-resistance wheat populations.