FUT8 is a critical driver of prostate tumour growth and can be targeted using fucosylation inhibitors

To identify signalling pathways altered by FUT8 in prostate cancer cells, we established luciferase-tagged CWR22Rv1 stable cell lines in which FUT8 was overexpressed (OE) and luciferase-tagged PC3 cell lines in which FUT8 was knocked down (KD). We then performed differential gene expression, Gene Ontology and GSEA analyses using data obtained from RNA-seq of the control and FUT8 overexpression, and the control and knockdown cell lines. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Lenti-ORF negative control CWR22Rv1 cells vs CWR22Rv1 cells with FUT8 overexpression, and from lenti shControl PC3 cells vs PC3 cells with FUT8 knockdown.