The ecotin-MASP affinities in Table 1 were measured at thermodynamic equilibrium as described in the Materials and methods. For tight-binding inhibition assays, enzyme and inhibitor concentrations nee
The Enzyme Kinetics Module from SigmaPlot 10 was used to evaluate the inhibition type and Ki values. A detailed statistical report was created for each binding model to compare different models and to
This Excel file contains initial velocity data for product formation in enzyme inhibition experiments. These datasets are used to generate the figures presented in the manuscript and supplementary not