Gene expression profiling in acute murine autoimmune hepatitis

The etiology of autoimmune hepatitis is poorly understood but likely involves Th1 cells producing IFN-gamma. BALB/c background TGF-beta1-/- mice rapidly develop fulminant Th1-mediated autoimmune hepatitis. Our aims are to profile liver gene expression in TGF-beta1-/- mice, to identify gene expression pathways dependent on IFN-gamma as possible targets for rational therapy, and to test potential targets directly in vivo in mice. Keywords: Comparative analysis of gene expression in livers of WT, TGFB1 & IFN knockout mice DNA microarray analyses were applied to liver RNA from TGF-beta1-/- mice, TGF-beta1-/- /IFN-gamma-/- mice, and TGF-beta1+/+ littermate controls. 3 mice from each group were analyzed in this study.自身免疫性肝炎的病因迄今尚未完全阐明，但其发病机制可能与分泌γ干扰素（IFN-γ）的Th1细胞相关。 BALB/c背景的转化生长因子β1（TGF-β1）基因敲除（TGF-beta1-/-）小鼠，可快速发生暴发性Th1细胞介导的自身免疫性肝炎。 本研究的研究目标为：对TGF-beta1-/-小鼠的肝脏基因表达谱进行分析，鉴定依赖于IFN-γ的基因表达通路以作为合理治疗的潜在靶点，并在小鼠体内直接验证这些潜在靶点。 关键词：野生型（WT）、TGFB1及γ干扰素（IFN-γ）基因敲除小鼠肝脏基因表达的比较分析 本研究采用DNA微阵列（DNA microarray）分析技术，对TGF-beta1-/-小鼠、TGF-beta1-/-/IFN-gamma-/-小鼠以及TGF-beta1+/+同窝野生型对照小鼠的肝脏RNA进行检测。本研究每组选取3只小鼠进行分析。