Gene expression profiling of renal medulla and cortex during postnatal development

The aim of this study is to establish a comprehensive transcriptome atlas that enables identification of key molecular pathways and morphogenic events regulating postnatal renal medulla/papillary and cortex development. To achieve this, a microarray expression profiling was performed on postnatal day 0-90 renal medulla and cortex obtained from CD1 male mice. Renal medulla and cortex were regionally dissected from postnatal day 0-90 CD1 male mice, and total RNA extracted for microarray expression profiling. Each time point consists of RNA pooled from 4 biological replicates, and an Agilent Bioanalyser test was performed to assess RNA integrity prior to sample pooling. The microarray data was analysed with the use of lumi and limma packages (Bioconductor) in R.本研究旨在构建一套全面的转录组图谱（transcriptome atlas），以识别调控出生后肾髓质/肾乳头及肾皮质发育的关键分子通路与形态发生事件。为达成该目标，本研究对从CD1雄性小鼠中获取的出生后第0至90天的肾髓质与肾皮质样本开展基因芯片表达谱分析。研究人员从出生后第0至90天的CD1雄性小鼠体内对肾髓质与肾皮质进行区域解剖分离，并提取总RNA用于基因芯片表达谱分析。每个时间点的RNA均来自4份生物学重复样本的混合，且在样本混合前已通过安捷伦生物分析仪（Agilent Bioanalyser）检测以评估RNA完整性。本研究采用R语言中Bioconductor（生物导体）平台的lumi与limma软件包对芯片数据进行分析。