single cell RNA-seq analysis of lung cancer cell lines treated with JTE-607

To assess the impact of CPA inhibition by JTE-607 on intra-cell-line transcriptomic heterogeneity, we profiled two lung cancer cell lines treated with DMSO or JTE-607 by 3' tag-based single-cell RNA-seq with 10x Chromium. For data analysis, we performed transcriptome quantification at the PAS-based transcript level to generate a PAS-by-cell count matrix, and then investigate the effect of JTE-607 treatment on PAS usage pattern and cellular states. Two different lung cancer cell lines were treated with DMSO or JTE-607. scRNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3′ kit version 2. To perform PAS-based transcript expression analysis, all samples were profiled using 3' sequencing.