Alignments of 18S,5.8S and 26S rDNA sequences of plants and fungal

The alignments were constructed using 18S, 5.8S and 26S rDNA sequences of all plants and fungal from GenBank on 9th February 2013. Multiple alignments of every group were conducted using MAFFT v6.864 (Katoh et al. 2005). The regions from NS7 priming site to the 3’ end of 18S, the 5’ end to LR3 priming site of 26S, and the complete 5.8S region were extracted for primer design and in silico tests. The "NonGreen plants" includes Rhodophytes and Glaucophytes. The "Basal fungi" includes Microsporidia and Cryptomycota.