RNAi efficiency in different tissues in rrf-1 mutants.

‘+’ and ‘−’ refer to the presence or absence of a phenotype in response to RNAi treatment in wild-type or rrf-1 animals. The rrf-1(pk1417), rrf-1(pk1417).4x (rrf-1(pk1417) four times outcrossed to WT(A) and rrf-1(ok589) mutants behaved similarly to RNAi targeting endogenous genes and are combined here as rrf-1. For strains expressing GFP-tagged transgenes, loss of GFP expression was assayed in response to gfp RNAi in the rrf-1(pk1417) mutant only.Phenotype abbreviations: Pro: proximal germ cell proliferation abnormal; Tum: tumorous germline; Prz: paralyzed, Unc/Twitcher: uncoordinated, sub-class, Twitcher; Gob: gut-obstructed; Clr: clear; Lva: larval arrest; Gro: slow growth; Ste: sterile; Bli: blistered; Dpy: dumpy; Bmd: body morphology defects; DTC: distal tip cell.1Citations refer to studies describing expression patterns and gene functions of the genes targeted by RNAi and to descriptions of the transgenes used;2Only the intestinal expression of daf-16p::gfp::daf-16 was examined;3The intestinal, pharyngeal, and gonadal expression of lgg-1p::gfp::lgg-1 was examined;4Data not shown;5RNAi phenotypes observed in 2nd generation;6Transgene expression was only partially reduced.