Validating compatibility of OsO4 staining with surface and intracellular staining by mass cytometry
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To test compatibility of OsO4 labeling in a mass cytometry experiment involving surface staining, methanol permeabilization and intracellular phospho-staining. Conclusion: OsO4 labeling can be incorporated either before or after surface/intracellualar staining and does not appreciably affect expression of any of the markers tested. For quality control, the acquisition event rate was maintained under 400 events/s, and the EQ beads were confirmed to have a median Eu151 intensity of over 1000 to ensure appropriate mass sensitivity.
创建时间：
2016-10-01
