Determining genes affected by ETV1 overexpression. Homo sapiens
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA732815
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Human cell line derived from normal-transformed prostate cells (RWPE1) were transfected with a plasmid vector (birA), either empty (vector control) or ETV1-spliced (ETV1 overexpression), and cultured for 7 days. cDNA libraries constructed from harvested RNA were sequenced and compared between vector control and ETV1 overexpression to determine transcriptional changes due to ETV1 overexpression. RWPE1 cells with STING [TMEM173] KO (CRISPR) were transfected with a plasmid vector (birA), either empty (vector control) or ETV1-spliced (ETV1 overexpression), and cultured for 7 days. cDNA libraries constructed from harvested RNA were sequenced and compared between vector control and ETV1 overexpression to determine transcriptional changes due to ETV1 overexpression (in the context of STING-KO).
创建时间:
2021-05-26



