Effects of BT-11 treatment on hippocampal gene expression in Female Fisher transgenic 344-AD rats. [Female RNAseq]

5 month old Female Fisher transgenic 344-AD (Tg-AD) rats expressing human Swedish amyloid precursor protein (APPsw) and Δ exon 9 presenelin-1 (PS1ΔE9) were treated with BT-11 (cat. no. HY-102013, MCE) at 8.5 mg/kg body weight administered in rodent chow. Following 6 months of BT-11 treatment, rats (11 months of age) were tested for cognitive behavior prior to sacrificing. Hippocampal Brain region were dissected and RNA sequencing (RNAseq) analyses was done on the Isolated sample. Taken together we showed that BT-11 treatment enriched pathways for females included passive transmembrane transporter activity, G protein-coupled receptor activity, G protein-coupled peptide receptor activity.本研究选用5月龄雌性Fisher转基因344-AD（Tg-AD）大鼠，该模型可表达人瑞典型淀粉样前体蛋白（APPsw）与Δ外显子9早老素1（PS1ΔE9）。将BT-11（货号HY-102013，MCE）以8.5 mg/kg体重的剂量掺入啮齿类动物饲料中对大鼠进行给药。经过6个月的BT-11给药处理后，对11月龄的大鼠开展认知行为学测试，随后实施安乐死。解剖分离海马脑区组织，对分离得到的样本进行RNA测序（RNA sequencing，RNAseq）分析。综合上述实验结果，我们证实BT-11给药可使雌性大鼠富集以下通路：被动跨膜转运蛋白活性、G蛋白偶联受体活性以及G蛋白偶联肽受体活性。