DNA methylation data of E, L1,L2 in generationd (G1-G4)

G1E were generation 1 queens reared from eggs transferred to queen cells on the 2nd day after laying. G1L1 were G1 queens reared from one-day old larvae transferred to queen cells on the 4th day after laying. G1L2 were G1 queens reared from two-day old larvae transferred to queen cells on the 5th day after laying. Eggs from the G1E queen were transferred as eggs to queen cells on the 2nd day post laying to create the G2E group. Eggs from the G1L1 queens were transferred to queen cells on the 4th day after laying to create the G2L1 group. Eggs from the G1L2 queens were transferred to queen cells on the 5th day after laying to create the G2L2 group. Queen cells of the G2 groups were treated the same way as the G1 queen cells. The emerging G2 queens were reared and sampled as for the G1 groups. We repeated this process to create the 3rd and 4th generation queen groups: G3E, G3L1, G3L2 and G4E, G4L1 and G4L2. Three types of queens were sampled in each generation, and 12 queen groups were sampled in total.