qPCR_COVID19_data.xlsx

Nasopharyngeal swabs from infected adults were obtained during the acute and late convalescent phase of SARS-CoV-2 infection and compared the expression of both genes with non-infected family household member contacts at the National University Hospital, Singapore. Viral inactivation was performed prior to RNA extraction, followed by cDNA synthesis using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA). RT-qPCR was performed to determine gene expression of ACE2 and TMPRSS2 against the housekeeping control 18S using the PerfeCTa SYBR® Green FastMix with Low ROX (Quantabio, Beverly, MA) and QuantStudio 5 Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA).