Shinya Inoue, Ted Inoue (2011) CIL:11938, Taraxacum officinale, pollen grain. CIL. Dataset

The movie shows several sequences illustrating the generation of 3D data using the CSU-10 confocal microscope to image the auto-fluorescence of acid treated pollen grains of Dandelion. The first sequence is a 'walk through' in the z direction with the raw images at left, and after unsharp masking on the left. The images in the stack were displaced sidewise sequentially, unsharp masked, summed to generate the views for different angles, and sequences of angular views shown. In these latter sequences, it appears as though 3D images of pollen grains are rotating, providing insight on the complex structure.

该影片展示了利用 CSU-10 共聚焦显微镜生成 3D 数据的多个序列，以捕捉经过酸处理的蒲公英花粉粒的自发光。首序列为 z 方向的‘穿越’过程，左侧展示原始图像，经左图去模糊处理后。图像堆栈中的图像依次进行横向位移、去模糊处理、汇总，以生成不同角度的视图，并展示角度视图序列。在这些后续序列中，花粉粒的 3D 图像仿佛在旋转，从而揭示了其复杂的结构。