Transcriptome analysis of lungs from six different mouse strains after infection with highly pathogenic H5N1 influenza A virus [set 2]

Susceptible (DBA/2J, 129/SvImJ, A/J) and Resistant (SM/J, C57BL/6J, Balb/cJ) mouse strain were inoculated with a highly pathogenic H5N1 influenza A virus (A/Hong Kong/213/2003) for 72 hours or not infected (control animals). Differences in expression were analyzed and used to identify candidate genes and pathways that contributed to the difference in H5N1 pathogenesis in these two groups of mice. Female 6-8 weeks old animals were inoculated with H5N1 virus or not and 72 hours later the lungs were obtained and immediately homogenized in Trizol. The extracted RNA was submitted for Illumina Gene expression profiling.将易感（DBA/2J、129/SvImJ、A/J）与抗性（SM/J、C57BL/6J、Balb/cJ）小鼠品系分别接种高致病性H5N1甲型流感病毒（A/Hong Kong/213/2003）并培养72小时，同时设置未感染的对照动物组。通过分析两组小鼠的基因表达差异，鉴定与H5N1在这两类小鼠中致病机制差异相关的候选基因及信号通路。本研究选用6~8周龄雌性小鼠，分为H5N1病毒接种组与未接种对照组，于接种72小时后摘取肺部组织，立即在Trizol试剂中进行匀浆处理；提取的总RNA用于开展Illumina基因表达谱分析。