H3K4me3 modifications and DNA methylation in microspore culture of Brassica rapa

Stress can induce the transformation of unicellular microspores in plants, shifting their developmental pathway from gametophyte formation to embryogenesis. This process ultimately leads to the production of haploid or double haploid plants. In this study, we explored the effects of heat stress on microspore culture by utilizing two contrasting varieties: the easily inducible K2 and the non-inducible 49 caixin, in combination with epigenetic analyses.