SETD1A is required for intestinal homeostasis and prevention of DNA damage

The impact of SETD1A on the small intestine was evaluated in sorted intestinal stem, transit amplifying (TA) and Paneth cells from Setd1aFC/+; Lgr5-eGFP-CreERT2/+ (control) and Setd1aFC/FC; Lgr5-eGFP-CreERT2/+ (knockout) mice, 4 days after tamoxifen-induced mutagenesis. Using 100-base-pair reads, over 35 million reads per sample with comparable unique mapped reads were obtained. To analyze differentially expressed genes, we applied DESeq2 analysis to the RNA-seq dataset. Analysis by DAVID and GSEA at a false discovery rate (FDR) of 5% was conducted.The stem and TA cell transcriptome revealed that Setd1a knockout cells exhibited a decreased expression of several DNA damage repair genes. Overall design: mRNA profiles of sorted stem, TA and Paneth cells from Setd1aFC/+; Lgr5-eGFP-CreERT2 (control) and Setd1aFC/FC; Lgr5-eGFP-CreERT2 (knockout) mice, 4 after tamoxifen were generated by deep sequencing.