Untargeted LC–MS/MS metabolomics of femoral/tibial bone tissues from OVX-induced osteoporosis mice treated with DCNPs, ALN, Mix, PBS treatment or healthy controls

This dataset contains untargeted metabolomics profiles acquired by UPLC–HRMS (positive and negative ESI modes) from femur/tibia bone tissues of OVX-induced osteoporosis mice across five groups.Healthy (sham) (Labelled NC in data file)OP + PBS (PBS)(Labelled OVX in data file)OP + Alendronate (ALN)(Labelled ALN in data file)OP + Mix (EA + ALN + PEI + CaCl2 without 2-FPBA)(Labelled Mix in data file)OP + DCNPs (EA + ALN + PEI + 2-FPBA + CaCl2) (Labelled NPs in data file)Female C57BL/6J mice were used. The goal is to characterize DCNP-mediated transcriptional reprogramming in bone, including activation of JAK2–STAT3 signaling and suppression of inflammatory lipid metabolism pathways identified in the companion manuscriptProviderUntargeted metabolomics was performed by Lianchuan Company (commercial service provider).Assay and platformAssay type: Untargeted UPLC–HRMS/MS (ESI+ and ESI−)UPLC: Waters ACQUITY UPLC with ACQUITY UPLC T3 column (100 × 2.1 mm, 1.8 μm), 40 °CMobile phases: A = water + 5 mM ammonium acetate + 5 mM acetic acid; B = acetonitrileGradient: 0–0.8 min 2% B; 0.8–2.8 min 2–70% B; 2.8–5.6 min 70–90% B; 5.6–6.4 min 90–100% B; 6.4–8.0 min 100% B; 8.0–8.1 min 100–2% B; 8.1–10.0 min re-equilibration at 2% BFlow rate: 0.3 mL/minMS: SCIEX TripleTOF 6600; IDA mode; mass range 60–1200 m/z; ESI+ 5000 V / ESI− −4500 V; source temp 500 °CQC: pooled QC injected every 10 samples; calibration every 20 samplesSamplesSpecies/strain/sex: Mus musculus, C57BL/6J, femaleTissue: Femur and tibia (bone)Groups and size: 5 groups × n = 6 biological replicates per group = 30 samples total (+ pooled QC)Model and treatments: variectomy (OVX) osteoporosis model; sham for HealthyEthics: Approved by the Laboratory Animal Ethics Committee of the First Affiliated Hospital of Wenzhou Medical University (No. WYYY-AEC-2025-008)