MiR-210 impact on the transcriptome suggests regulation of inflammation and regeneration pathways

In order to understand the consequences of miR-210 blocking on the ischemia response, the transcriptomic changes were investigated by microarray technology in gastrocnemius muscles of ANTI-210 and SCR treated mice, 7 days after ischemia. Identified differentially expressed genes were used to perform gene-ontology enrichment-analysis, allowing to identify several terms falling into the biological macro areas of angiogenesis and blood vessel development, as well as related categories, such as cell adhesion, migration and proliferation. These findings are in agreement with the identified positive role played by miR-210 in the neo-vascularization process. Furthermore, many terms were also related to metabolism and mitochondrial organization, in keeping with the role of miR-210 in the regulation of oxidative phosphorylation. MiR-210 was blocked in ischemic gastrocnemius muscles by injecting ANTI-210 LNA-GAPmers (Exiqon) and transcriptomic changes were measured by lllumina BeadChip platform.