Experimental data on naloxone supplementation during vitrification of equine in vitro matured oocytes after overnight holding: insights from a comparative study with the bovine model

This dataset contains the raw data from the two experiments: i) bovine oocyte IVM and viability dataset refers to Exp. 1 in which bovine immature (bGV) and mature (bMII) bovine oocytes were vitrified without (VIT) or with NX (VIT-NX) to assess protocol efficiency and evaluate whether results obtained in bovine MII oocytes (without holding) could serve as a counterpart to equine oocytes subjected to overnight holding; ii) equine oocyte viability and cleaved embryos after ICSI, equine oocyte GSH, ROS, and HMMP levels, and equine oocytes qRT-PCR datasets refer to Exp. 2 in which equine in vitro matured oocytes after overnight holding (eMII) were vitrified with or without NX, and analyzed for viability, reactive oxygen species (ROS), glutathione (GSH), high mitochondrial membrane potential (HMMP), developmental competence following intracytoplasmic sperm injection (ICSI), and expression of apoptosis-related genes (BCL2, BAX, p53) by quantitative real-time PCR (qRT-PCR).