CD4 Fluorochrome stain index in Aurora 5L - Immunotrol vs Fresh human Blood

Fluorochrome brightness via CD4 stain indexes in Aurora 5L. Brightness comparison of Beckman Coulter Immuno-TROL and fresh human blood.
Conclusion:
Stain Index (and fluorochrome brightness) is inversely proportional to carrier autofluorescence levels.
Notes:
All samples were acquired in Cytek Aurora 5L (UV16-V16-B14-YG10-R8) at Cytek recommended assay settings. All FCS are raw files acquired after successful instrument embedded QC. Immuno-Trol samples are labelled as "Imt", fresh human blood samples are labelled as "huB". Samples were stained with 26 unique fluorochromes conjugated to CD4 antibody (clone SK3; BD Horizon evaluation kit cat #566352, Becton Dickinson, Franklin Lakes, NJ, USA). Experiments done after successful QC in Cytek Aurora 5L