Sequences, accession numbers and tissue distribution of the peptides characterised in D. radicum larvae.

a)Leu and Ile have the same molecular mass. Since we did not obtain distinguishing high-energy collision w-fragments [76], we are unable to distinguish between these two amino acids. Therefore, Leu and Ile in the sequences above have to be considered as predicted only based on the homolog peptides from Drosophila or other Dipterans. Small letter c within a sequence indicates cysteines that form an intramolecular disulfide bridge.b)tPSO = thoracic PSO, aPSO = abdominal PSO, data from direct profiling of the dorsal sheath of the adult thoracico-abdominal ganglion.c)these peptides could be sequenced in their SPITC-labelled form.d)Mass peak indicative of this peptide appeared consistently, but could not be fragmented. Sequence adapted from [9].e)a peptide with similar mass but different sequence (SPKQDFMRFa, 1154.6 Da and KPNQDFMRFa, 1181.6 Da) was reported by Audsley et al. [9].f)The y9-fragment identifying the sequence order of positions 2–3 could not be found in SPITC-labelled and unlabeled spectra. The sequence LG is assumed since a very similar tachykinin (Cav-TKII: GLGNNAFVGVRa) was isolated and Edman-sequenced from the blowfly Calliphora vomitoria[73].g)Only amino acids 1–11 of APK have been fully fragmented and are sequence identical to the N-terminus of APK of Drosophila melanogaster[24], [28]. The y-fragment representing amino acids 12–16 matches the mass of amino acids 12–15 of Drosophila APK plus the mass of tyrosine. Therefore, we assume the listed sequence. The position of the tyrosine and the C-terminal NAPK is not confirmed by fragmentation data.