In vivo atlas of RNA secondary structures recognized by ADARs [irCLASH-seq&hiCLIP-seq]

We performed irCLASH and HiCLIP for ADAR1-3 in HEK293 cells. In addition, we performed mRNA-seq in ADAR1-3 overexpressed HEK293 cells and control HEK293 cells Overall design: Three ADAR genes are present in the human genome. ADAR1 and ADAR2 bind to dsRNA and catalyze adenosine-to-inosine RNA editing, whereas ADAR3 is thought to be catalytically inactive and predominantly acts as an inhibitor of editing. Despite the importance of ADARs in a variety of regulatory processes, little is known about the in vivo structures of their RNA substrates. Therefore we developed irCLASH to generate the in vivo atlas of RNA secondary structures recognized by ADARs in this study.