Next Generation Sequencing of PKD2 mutant mice

Purpose: Determine the differential gene expression pattern between wildtype, Pkd2-KO and Pkd2-miR-214 KO mice Methods: kidney mRNA profiles of Pkd2-KO and Pkd2-mir-214-KO mice was sequenced with N of 3 in each group Results: 972 differentially expressed transcripts were identified between Pkd2-KO kidneys and Pkd2-miR-214-KO kidneys Conclusion: Deletion of miR-214 promotes interstitial inflammation in mouse models of ADPKD kidney mRNA profiles of Pkd2-KO and Pkd2-miR-214 KO were determined by polyA mRNA sequencing with an N=3 different biological replicates in each group研究目的：明确野生型、Pkd2基因敲除（Pkd2-KO）与Pkd2-miR-214基因敲除（Pkd2-miR-214 KO）小鼠之间的差异基因表达模式。研究方法：采用聚腺苷酸信使RNA（polyA mRNA）测序技术，对每组各3个生物学重复的Pkd2-KO与Pkd2-miR-214 KO小鼠的肾脏mRNA转录组进行测序分析。研究结果：在Pkd2-KO小鼠肾脏与Pkd2-miR-214 KO小鼠肾脏之间，共鉴定出972个差异表达转录本。研究结论：在常染色体显性遗传性多囊肾病（Autosomal Dominant Polycystic Kidney Disease, ADPKD）小鼠模型中，敲除miR-214可促进肾脏间质炎症反应。本研究通过聚腺苷酸信使RNA测序技术，对Pkd2-KO与Pkd2-miR-214 KO小鼠的肾脏mRNA转录组进行了测定，每组设置3个独立生物学重复。