Characteristics of primers and primer pairs.
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*f = forward, r = reverse;**melting temperatures (Tm) of oligonucleotide sequences were calculated using Oligo Analysis Tool (http://www.operon.com/tools/oligo-analysis-tool.aspx),***percentage values estimated from the total cloning/sequencing approach adopted in other biodiversity studies on sampled forest soils (ITS: 567, Mix5: 986, Mix7: 1117 sequences).
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2015-12-02
