Genome wide methylation analysis of aged uterus and decidua

We use MeDIP-sequencing to measure global DNA methylation (5mC) in the uteri of young and aged C57BL/6 mice at day 3.5 (E3.5) of pregnancy, and of young and aged decidua at day 11.5 (E11.5) of pregnancy For E3.5 uterus MeDIP-seq data, we generated biological replicates from 3 young (8-20 week old) and 5 aged (41-49 week old) mice. For E11.5 data, we generated biological replicates of 6 deciduae from 3 litters (2 from each litter) from young mothers (9-10 weeks, n=3) and 10 deciduae from 5 litters of aged mothers (43-47 weeks, 2 deciduae from each litter). Aged E11.5 samples were split into 2 groups: those accompanying embryos that appeared normal (An, n=3) and those with morphologically abnormal embryos (Aa, n=3). One An and one Aa decidua was taken from each litter我们采用甲基化DNA免疫沉淀测序（MeDIP-sequencing）技术，检测妊娠第3.5天（E3.5）时年轻与年老C57BL/6小鼠子宫，以及妊娠第11.5天（E11.5）时年轻与年老蜕膜组织中的全基因组DNA甲基化（5mC）水平。针对E3.5子宫的MeDIP测序数据，我们分别从3只年轻（8~20周龄）、5只年老（41~49周龄）小鼠中获取生物学重复样本。针对E11.5样本，我们从3窝年轻母鼠（9~10周龄，n=3）中获取6份蜕膜组织（每窝2份）作为生物学重复；同时从5窝年老母鼠（43~47周龄，每窝采集2份蜕膜组织）中获取10份蜕膜组织作为生物学重复。年老组的E11.5样本被分为两组：伴随形态正常胚胎的蜕膜（An组，n=3），以及伴随形态异常胚胎的蜕膜（Aa组，n=3），每窝分别采集1份An组与1份Aa组蜕膜组织。