34f0bde1-41b1-4578-931f-402fd4b3fd7a - samples
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下载链接：
https://www.omicsdi.org/dataset/ega/EGAD00001011155
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资源简介：
This dataset contains raw FASTQ files from single cell RNA sequencing of SarBC-01 cells treated with Dexamethasone vs DMSO, with or without Matrigel, processed with MULTI-seq. Cells from 4 different culture conditions (+Mat/Dex, +Mat/DMSO, -Mat/Dex, -Mat/DMSO) were harvested, processed for multiplexing using the MULTI-seq protocol and loaded in a Chromium Single Cell 3ʹ GEM Library and Gel Bead Kit v3 (10x Genomics). Gene expression (cDNA) and MULTI-seq libraries were prepared according to the manufacturers’ protocol of Chromium Next GEM Single Cell 3’ reagents Kits v3.1 (Dual Index). Finally, cDNA and MULTI-seq libraries were analyzed using an Agilent Bioanalyzer (DNA High Sensitivity kit) and sequenced on a NovaSeq6000 (S2 flow cell) platform.
MULTISEQ BARCODES:
TTAGCCAG => Matrigel/DMSO
CCACAATG => Matrigel/Dexamethasone
GCACACGC => noMatrigel/DMSO
AGAGAGAG => noMatrigel/DexamethasoneEGA dataset EGAD00001011155
创建时间：
2024-02-28
