Next generation sequencing of human monocyte derived dendritic cells stimulated with MGL-stimulating glycodendrimers in the absence or presence of LPS

To investigate the effect of MGL ligation in monocyte-derived dendritic cell biology, we generated control dendrimers and two different glycodendrimers exposing the MGL ligands aGalNAc or GalNAcß1-4Gal. aGalNAc and GalNAcß1-4Gal glycodendrimers were validated in the corresponding manuscript. Monocyte-derirved dendritic cells were generated by culturing monocytes for 4 days with IL-4 and GM-CSF. To study the effect of MGL-ligation at the transcriptional level, next generation sequencing was performed on monocyte-derived dendritic cells from three independent donors, stimulated for 4 h with control, aGalNAc or GalNAcß1-4Gal glycodendrimers in the absence or presence of LPS. Overall design: 18 samples were included in next generation sequencing, including triplicates for control, aGalNAc and GalNAcß1-4Gal glycodendrimers, all-in the absence or presence of LPS. The control dendrimers and control dendrimers in the presence of LPS serve as reference samples