Fucci_mESC_timelapse_imaging

Live-cell Imaging Data of Fucci-labeled mESCs Dataset Overview This dataset contains live-cell imaging data of mouse embryonic stem cells (mESCs) expressing Fucci cell cycle reporters. Includes raw timelapse images and quantitative fluorescence measurements. File Description Image Files: Fucci_mESC_timelapse_bio_rep1.tif - Timelapse imaging, replicate 1 (50 hours, 10-minute intervals)Fucci_mESC_timelapse_bio_rep2.tif - Timelapse imaging, replicate 2 (50 hours, 10-minute intervals) Measurement Files: Fucci_mESC_timelapse_bio_rep1.csv - Raw fluorescence measurements, replicate 1Fucci_mESC_timelapse_bio_rep2.csv - Raw fluorescence measurements, replicate 2Column Definitions Label - Cell identifier using lineage tracking system:Base number indicates original cell (e.g., 1)M suffix indicates mitotic cell (e.g., 1M)Decimal notation tracks daughter cells across divisions:First division: 1.1, 1.2Second division: 1.1.1, 1.1.2, 1.2.1, 1.2.2Example lineage: Cell 1 → mitosis (1M) → daughters 1.1 and 1.2 POSITION_X - X-coordinate position (pixels)POSITION_Y - Y-coordinate position (pixels)FRAME - Timelapse frame numberRADIUS - Tracking circle radius (fixed at 5 pixels for all cells)MEAN_INTENSITY_01 - Mean mVenus fluorescence intensity (S/G2/M marker)MEAN_INTENSITY02 - Mean mCherry fluorescence intensity (G1 marker) Script:live_imaging_analysis.R - R script used to generate figure S1D-G