The transition from quiescent to activated states in human hematopoietic stem cells is governed by dynamic 3D genome reorganization [ChIP-Seq]

We profiled chromatin accessibility in sorted hematopoietic populations. We used this to identify signatures of chromatin accessibility which discriminated LT-HSC from other hematopoietic stem and progenitor cell populations, discriminated by CTCF binding sites. We further validated this with RNA-Seq on sorted cord blood populations following shCTCF, and Hi-C and CTCF ChIP-Seq on the OCIAML-2 cell line. CTCF ChIP-Seq in the cell line OCIAML-2 Note from submitter: Raw data has been submitted separately to the EGA since it contains patient-sensitive information