Machine Learning Identifies Signatures of Macrophage Reactivity and Tolerance

A continuum of macrophage polarization states is essential tissue homeostasis. We used machine learning approaches to identify universally relevant definition of macrophage polarization states and create a predictive framework for developing macrophage-targeted precision diagnostics and therapeutics. CCDC88A was identified as a key gene in the continuum state clusters that is essential for the tolerant polarization state. Overall design: Murine peritoneal macrophage were isolated from macrophage specific CCDC88A (GIV) knockout mice and their littermate WT controls 4 days after thiglycollate injection (IP). Peritoneal macrophage were stimulated or not with 100ng/ml of LPS for 6h (n=3)