Analysis of random clones at various stages of library construction.
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Randomly selected clones were analyzed from (A) MTBLIB25 library (100–300 bp) and (B) MTBLIB27 library (300–800 bp) at various stages of library construction: 1, transformants obtained after large-scale electroporation of the ligation sample; 2, transductants obtained after infection of TOP10F’ cells with the primary phage library; 3, transductants obtained after infection of TOP10F’ cells with the trypsin-treated primary phages; 4, transductants obtained after infection of TOP10F’ cells with the secondary ORF-selected phage library.
*Percentage of recombinant clones that aligned to the M. tuberculosis (M. tb) genome.
†Percentage of clones in-frame with the PelB signal sequence (PelBss) and gIIIP in the phagemid.
‡Percentage of total in-frame clones (as in III) that aligned with the M. tb proteome (genic ORFs).
Number of positive clones/total clones analyzed is given in brackets.
创建时间：
2013-09-27
