Analysis of random clones at various stages of library construction.

Randomly selected clones were analyzed from (A) MTBLIB25 library (100–300 bp) and (B) MTBLIB27 library (300–800 bp) at various stages of library construction: 1, transformants obtained after large-scale electroporation of the ligation sample; 2, transductants obtained after infection of TOP10F’ cells with the primary phage library; 3, transductants obtained after infection of TOP10F’ cells with the trypsin-treated primary phages; 4, transductants obtained after infection of TOP10F’ cells with the secondary ORF-selected phage library. *Percentage of recombinant clones that aligned to the M. tuberculosis (M. tb) genome. †Percentage of clones in-frame with the PelB signal sequence (PelBss) and gIIIP in the phagemid. ‡Percentage of total in-frame clones (as in III) that aligned with the M. tb proteome (genic ORFs). Number of positive clones/total clones analyzed is given in brackets.