RNA-seq of cardiac lineage cells (day 3 of cardiac differentiation) derived from human embryonic stem cells (hESCs) overexpressed with Monkeypox virus-protein revealed the effects of H3L and A29L in human cardiac development.

Monkeypox virus (MPV) can infect human cells and induces injuries in human. However, whether and how MPV affect the function of human cells remain to be elucidated. Here, we overexpressed H3L and A29L in hESCs, separately. We appliced RNA-seq on cardiac lineage cells (day 3 of cardiac differentiation) derived from human embryonic stem cells (hESCs) overexpressed with Monkeypox virus-proteins (Control, H3L and A29L) and want to know gene expression patterns of control and overexpression cell lines. Subsequent data analyses will help us to know whether and how H3L and A29L affect human cardiac development. The stable hESCs overexpressed with H3L or A29L were rotinely maintained in mTesR1 medium in 6-well plate. For cardiac differentiation, STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (STEMCELL Technologies) was used according to the manual. The hESCs were differentiated into cardiac lineage cells. Then, the cardiac lineage cells on day 3 post differentiation were collected for bulk RNA-seq. Total RNAs were extracted and purified with RNeasy Mini Kit (Qiagen). Two biological replicates were applied for RNA-seq.