Efficacy of benzo[cd]azulenes as Pim-1 inhibitors in vitro and in cell-based assays.

1Prepared according to [28].2Prepared according to [29].3Prepared according to [30].Residual in vitro activity of Pim-1 was determined in the presence of 10 µM concentrations of benzo[cd]azulenes dissolved in DMSO. Data were calculated as the percentage of Pim-1 autophosphorylation as compared with DMSO-treated controls. Residual cellular viabilities were determined by the MTT assay from FD/Neo and FD/Pim44 cells that had been cultured for 24 h without IL-3 in the presence of 0.1% DMSO or 5 µM inhibitors. Data were calculated as the percentage of viable cells in treated cultures as compared with DMSO-treated controls from at least two independent experiments with duplicate samples. N.D. means that viability was not determined.